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产品货号:ZZK-K-ASPTM
产品品名:阿斯巴甜检测试剂盒
英文品名:Aspartame Assay Kit
规格型号: 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
A simple and reliable method for the specific determination ofaspartame in beverages and foodstuffs.
INTRODUCTION:
Aspartame is a dipeptide sweetener comprising L-aspartate and
methyl esterified L-phenylalanine (Asp-Phe-O-Me). At 200 times the
sweetness of sucrose, and with a structure similar to dipeptides found
in a normal diet, its use in low calorie carbonated drinks and other
foodstuffs has been widely accepted since the early 1980s. However,
due to its L-phenylalanine content, consumption of this compound
must be strictly monitored by those suffering from phenylketonuria.
There is also a significant level of concern voiced by some who
believe this sweetener poses serious health problems relating to the
three components, L-aspartic acid, L-phenylalanine and methanol,
though there is, as yet, no evidence to support such claims.
KITS:
Kits suitable for performing 50 assays in manual format (or 500
assays in auto-analyser format or 500 assays in microplate format) are
available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (12 mL, pH 8.0) plus 2-oxoglutarate and
sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: (x2) NADH plus stabiliser.
Stable for > 5 years at -20°C.
Bottle 3: L-Malate dehydrogenase plus glutamate-oxaloacetate
transaminase suspension (1.1 mL).
Stable for > 2 years at 4°C.
Bottle 4: Peptidase M suspension (1.1 mL).
Stable for > 2 years at 4°C.
Bottle 5: Aspartame control powder (~ 1 g, ~ 2.5% w/w
aspartame on an “as is” basis. The exact
concentration of aspartame is given on the vial
label).
Stable for > 5 years when stored dry at 4°C.
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