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麦芽淀粉酶检测试剂盒ZZK- K-MALTA
产品货号:ZZK- K-MALTA
产品品名:麦芽淀粉酶检测试剂盒
英文品名:Malt Amylase Assay Kit
规格型号:100 assays per kit
α-淀粉酶,β-淀粉酶在淀粉降解代谢过程中或发酵糖发芽或谷物麦芽中发挥重要作用。
上海甄准生物提供麦芽淀粉酶检测试剂盒,比色法(400nm)测定谷物、麦芽、食品、饮料和发酵产品中的α-淀粉酶和Beta-淀粉酶。
原理:
(1) α-淀粉酶检测利用“Ceralpha” 方法
(2) Beta-淀粉酶检测利用 “Betamyl-3” 方法
反应时间:
~ 20 min (Ceralpha 方法)
~ 10 min (Betamyl-3 方法)
检测限:0.05 U/mL
适用样品:面粉、麦芽、发酵液和其他原料
优点:
l 价格低廉(每次检测成本)
l 所有试剂配制后的稳定性>2年
l 只适用于酶法试剂盒
l 特异性高
l 方法简单
l 包含标准品
l 方法认证:AOAC (Method 2002.01), AACC (Method 22-02.01),ICC (Standard No. 303), RACI (Standard Method), and CCFRA (Flour Testing Working Group Method 0018)
l RACI (标准方法)
REFERENCES:
1. McCleary, B. V. & Sheehan, H. (1987). Journal of Cereal Science, 6, 237-251.
2. Sheehan, H. & McCleary, B. V. (1988). Biotechnology Techniques, 2, 289-292.
3. McCleary, B. V., McNally, M., Monaghan, D. & Mugford, D. C. (2002).Measurement of α-amylase activity in white wheat flour, milled malt, and microbial enzyme preparations using the Ceralpha Assay: collaborative study.J. AOAC International, 85, 1096-1102.
4. Mathewson, P. R. & Seabourn, B. W. (1983). Journal of Agriculture and Food Chemistry, 31, 1322-1326.
5. McCleary, B. V. & Codd, R. (1989). Journal of Cereal Science, 9, 17-33.
6. Erdal, K., Jensen, M. O., Kristensen, M., Krogh, J. J., Riis, P. & Vaag, P., E. B. C. Proceedings of the 24th European Brewery Convention Congress, Oslo, 1993, 147.
7. Santos, M. M. & Riis, P. (1996). Journal of the Institute of Brewing, 102, 271-275.
Acknowledgment:
Special thanks are due to Dr. Finn Lok, Carlsberg Research Centre and QA, Copenhagen for assistance in finalising the described assay formats and booklet.
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